Beilstein J. Org. Chem.2024,20, 1–11, doi:10.3762/bjoc.20.1
, Bunkyo-ku, Tokyo 113-8657, Japan 10.3762/bjoc.20.1 Abstract Recently, we identified the biosynthetic gene cluster of avenalumicacid (ava cluster) and revealed its entire biosynthetic pathway, resulting in the discovery of a diazotization-dependent deamination pathway. Genome database analysis revealed
reaction enabled us to perform a kinetic analysis of AvaA7, which confirmed that AvaA7 catalyzes the denitrification of 3-diazoavenalumic acid in avenalumicacid biosynthesis. This study deepened our understanding of the highly reducing type II polyketide synthase system as well as the diazotization
-dependent deamination pathway for the production of avenalumicacid or p-coumaric acid.
Keywords: actinomycetes; avenalumicacid; biosynthesis; p-coumaric acid; polyketides; Introduction
The genomes of microorganisms possess diverse biosynthetic gene clusters (BGCs) to produce natural products [1]. In
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Graphical Abstract
Figure 1:
Comparison of ava and cma clusters and the biosynthetic pathway of p-coumaric acid. A) Schematic re...